赛默飞世尔Thermo Fisher X1344 Unive
rsal refill starter kit Finntip 250 0.5µL to 250µL Finnpipette yellow 52mm 货号：T_70311855863- ￥1000 - 20000
- Thermo Fisher
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- T_70311855863
- 2025年07月11日
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- 文献和实验
- 技术资料
- 库存：
999
- 供应商：
北京泽平
- 现货状态：
热销品大量现货，其余请咨询
- 保修期：
1年
- 规格：
EA
货号：T_70311855863
中文名称：
英文名称：X1344 Universal refill starter kit Finntip 250 0.5µL to 250µL Finnpipette yellow 52mm
货期：热销品现货，其他请咨询
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文献和实验Southern Hybridization Protocols
solution is the same. 60 ℃ for lower stringency Southern hybridization. 65℃ for higher stringency Southern hybridization. For probe labelling: Use RadPrime DNA Labeling System (Invitrogen) Denature 25 ng (1 µ l) in 19 µ l of sterile
RNase Protection Assay（RPA)操作步骤
4 µl 5X transcription buffer 1 µl RPA Template Set 10 µl [a-32P]UTP 1 µl T7 RNA polymerase (Keep at -20°C until use, return to -20°C immediately). Mix by gentle pipetting or flicking and quick spin in a microfuge. Incubate
to the column and wash the reaction vial with an additional 200-300 µl of fresh TEAB. Add the wash to the column and allow it to begin to run down into the matrix. When the dye product has been absorbed into the matrix, refill the column with buffer and reattach
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