赛默飞世尔Thermo Fisher Manual syri

小RNA的Northern BLOT

#______________  1 μl T4 Polynucleotide Kinase, 10 U/μl Lot#______________  X μl Nuclease free H2O to 10 μl  37℃ at least 1 hr  After incubation, bring reaction volume up to 50 μl before cleaning up with spin column

The In Situ PCR:Amplification and Detection in a Cellular Context

were then deposited by cytocentrifugation (450 rpm x 5 minutes, Shandon cytocentrifuge) onto two glass slides. Viral RNA was digested with ribonucleases A (100 µg/ml) and T1 (10 µg/ml), and the cells were postfixed for two hours in 4% paraformaldehyde to improve

原位PCR的详细介绍

were performed in identical fashion. 2. Tissues on Slides. Deparaffinized tissue sections were overlaid with standard PCR cocktail (20-40µl, depending on the size of the section) containing 1 µM of the multiple primer set. The reaction mixture also contained 10