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- 文献和实验
- 技术资料
- 库存:
999
- 供应商:
北京泽平
- 现货状态:
热销品大量现货,其余请咨询
- 保修期:
1年
- 规格:
EA
货号:T_70311307881
中文名称:
英文名称:X6 Volumetric flask Corning class B reusable flat bottom polypropylene with 10/19 tapered PP stopper
货期:热销品现货,其他请咨询




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文献和实验the pellet in 10 ml sterile 1 X TE/LiAC (made fresh from stocks of 10 X TE [0.1 M Tris-HCI, 0.01 M EDTA, pH 7.5] and 10X LiAC [1 M lithium acetate adjusted to pH 7.5 with dilute acetic acid]. Repeat. Pellet cells in microfuge at 7000rpm, discard sup
out quicker, so make sure incubator is humid. I had the Yale machine shop make metal holders that hold 3 deepwell boxes each. I attached these to the flat disc of a vertical rotating wheel (Glas-Col rugged rotator, from Fisher). The boxes thus get rotated
Isolation, Culture, and Differentiation of Progenitor Cells from the Central Nervous System
in Step 20) Plates, polystyrene, 96-well flat bottom, low cell binding (Corning 3474) (optional; see Step 21) Razor blade, sterile Scissors, sterilized microspring (8.5-cm) (Fine Science Tools 15009-08) Slides, eight-well chamber
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