赛默飞世尔Thermo Fisher X12 BOTTLE
TOP FILTER,NALGENE, MEMBRANE DIA.50MM, FILTER SIZE 150ML, FITS BOTTLE NECK SIZE 45MM, 货号：T_70310319590- ￥70 - 1400
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- T_70310319590
- 2025年07月11日
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- 库存：
999
- 供应商：
北京泽平
- 现货状态：
热销品大量现货，其余请咨询
- 保修期：
1年
- 规格：
EA
货号：T_70310319590
中文名称：
英文名称：X12 BOTTLE TOP FILTER,NALGENE, MEMBRANE DIA.50MM, FILTER SIZE 150ML, FITS BOTTLE NECK SIZE 45MM,
货期：热销品现货，其他请咨询
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文献和实验Membrane Transfer and Crosslinking for RNA
The composition of the transfer buffer is usually a 5X SSC/10 mM NaOH solution. These mildly alkaline conditions shear the RNA into smaller fragments and denature it as it is deposited onto the membrane. A brief protocol for assembly (see Figure 1
[分享] Strip Antibodies and Re-use Protein blots membrane
10min in PBST 5. Transfer the membrane to strip buffer (preheated to 55-65C) for 20min, 6. Repeat step 5 once 7. Rinse 3X in PBST followed by 10min in PBST 8. Check strip efficiency (normally, it is OK, but if the expression is too high
in methanol at room temp. 3. Remove methanol and add 1x Blotting buffer until ready to use. G. Membrane transfer 1. Assemble "sandwich" for Bio-Rad's Transblot. 2. Prewet the sponges, filter papers (slightly bigger than gel) in 1x Blotting buffer
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