赛默飞世尔Thermo Fisher X12 BOTTLE
TOP FILTER,NALGENE, MEMBRANE DIA.50MM, FILTER SIZE 150ML, FITS BOTTLE NECK SIZE 45MM, 货号：T_70310319590- ￥70 - 1400
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- 2025年07月11日
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- 库存：
999
- 供应商：
北京泽平
- 现货状态：
热销品大量现货，其余请咨询
- 保修期：
1年
- 规格：
EA
货号：T_70310319590
中文名称：
英文名称：X12 BOTTLE TOP FILTER,NALGENE, MEMBRANE DIA.50MM, FILTER SIZE 150ML, FITS BOTTLE NECK SIZE 45MM,
货期：热销品现货，其他请咨询
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文献和实验Membrane Transfer and Crosslinking for RNA
The composition of the transfer buffer is usually a 5X SSC/10 mM NaOH solution. These mildly alkaline conditions shear the RNA into smaller fragments and denature it as it is deposited onto the membrane. A brief protocol for assembly (see Figure 1
in methanol at room temp. 3. Remove methanol and add 1x Blotting buffer until ready to use. G. Membrane transfer 1. Assemble "sandwich" for Bio-Rad's Transblot. 2. Prewet the sponges, filter papers (slightly bigger than gel) in 1x Blotting buffer
[分享] Strip Antibodies and Re-use Protein blots membrane
10min in PBST 5. Transfer the membrane to strip buffer (preheated to 55-65C) for 20min, 6. Repeat step 5 once 7. Rinse 3X in PBST followed by 10min in PBST 8. Check strip efficiency (normally, it is OK, but if the expression is too high
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