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- 库存:
999
- 供应商:
北京泽平
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热销品大量现货,其余请咨询
- 保修期:
1年
- 规格:
EA
货号:T_70312803858
中文名称:
英文名称:Tube UVP XX-series 254nm shortwave length 1220mm 40W for use with XX-series bench lamp
货期:热销品现货,其他请咨询




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文献和实验PCR引物设计和优化(PCR PRIMER DESIGN AND REACTION OPTIMISATION)
concentration (>1nM), and when dNTP and / or primer depletion may become limiting. Reaction Buffer Recommended buffers generally contain : 10-50mM Tris-HCl pH 8.3, up to 50mM KCl, 1.5mM or higher MgCl2, primers 0.2 - 1uM each primer, 50 - 200uM each dNTP
Monoclonal Antibody Production Protocol
to a 15ml conical tube and let the cell debris settle out (approx. 5 min.). Remove the cell suspension (without disturbing the settled cell debris) and transfer to a 50ml conical tube. Add an additional 30ml of IMDM and pellet the cells at 1200 rpm
, set up a second series of dilutions, this time diluting by 1/2 each time. That is, transfer 5.0 ml of the 10 culture to 5.0 ml of fresh broth and mix thoroughly. Use 5.0 ml of this and transfer to 5.0 ml of fresh broth to produce a 1/4 dilution. Repeat
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