赛默飞世尔Thermo Fisher X2 Earth wi

Detection and Analysis of Protein‐Protein Interactions of Organellar and Prokaryotic Proteomes by Blue Native and Colorless Native Gel Electrophoresis

cell (Bio‐Rad) or SE 600 ruby dual cooled vertical unit (Hoefer) with clamps, glass plates, casting unit, and buffer chambers Thick spacers: 3‐mm spacers for the Bio‐Rad apparatus or for the Hoefer unit, conventional

Methods for DNA sequencing

of loading dye. When double-stranded pUC-based subclones are used as templates, the amount of primer is doubled and a denaturing/annealing step is added. Here, 3 μg of plasmid DNA, isolated by either the mini- or midi-prep diatomaceous earth method, is mixed

Methods for DNA sequencing

. Optically-ground, low fluorescence glass plates are carefully cleaned with hot water, distilled water, and ethanol to remove potential fluorescent contaminants prior to taping. Denaturing 6% polyacrylamide gels are poured into 0.3 mm X 89 cm X 52 cm taped