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- 文献和实验
- 技术资料
- 库存:
999
- 供应商:
北京泽平
- 现货状态:
热销品大量现货,其余请咨询
- 保修期:
1年
- 规格:
EA
货号:T_70312673125
中文名称:
英文名称:PH ELECTRODE EUTECH GEL FILLED SINGLE JUNCTION plastic body 12mm x 110mm length with 1m cable
货期:热销品现货,其他请咨询




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文献和实验Ex Ovo Electroporation of DNA Vectors into Pre-gastrulation Avian Embryos
. Approximately 2 mm from the end, bend the wire at a 45° angle (see Fig. 1 , top electrode). Using clear nail polish, insulate the wire near the junction where the cathode will be mounted on the micromanipulator. Couple the cathode to a stainless steel rod
round plastic coverslips, sterile (e.g., Thermanox; Fisher) Microscope slides 50‐ml plastic graduated cylinders cut to 30‐ml mark
(for MBP or fusion proteins) and polymerize as usual. 2) For whole cell extracts, run 10-50μg protein per lane, or run 50-100% of an IP. 3) Wash gel twice for 30min each with 150-200mls 20% isopropanol in buffer A (50mM HEPES pH 7.4, 5mM b-ME)[2].
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