赛默飞世尔Thermo Fisher Shaft stirr

Stripping Western Blots

IP 3X with lysis buffer and once with 1X kinase buffer (50mM HEPES or Tris pH 7.4, 10mM MgCl2, 10mM MnCl2, 1mM DTT) 3) Prepare Reaction Mix (use 40μl per IP): (per IP) 20μl 2X Kinase Buffer 0.6μl 1mM ATP (final [ ] = 15μM)

Biotinylation of a Propargylated Cyclic (3′‐5′) Diguanylic Acid and of Its Mono‐6‐Thioated Analog Under “Click” Conditions

(coarse porosity, Kontes) 4‐mL screw‐cap glass vials (Wheaton) 5‐µm Supelcosil LC‐18S HPLC column (25 cm × 10 mm; Supelco

Convenient and Efficient Approach to the Permanent or Reversible Conjugation of RNA and DNA Sequences with Functional Groups

Rubber septa for 14/20‐ and 24/40‐glass joints (Aldrich) Magnetic stirrer and stir bars (VWR) 4‐mL screw‐cap