赛默飞世尔Thermo Fisher 1725 TLL 25

小RNA的Northern BLOT

. Rinse with distilled water; double distilled water and ethanol.  Put 1.5 mm thick spacers on the 20 x 40 cm glass plate, put the notched glass plate on top of the spacers. Clamp the plates together on one side, top and bottom with large binder

Encapsulation of Cardiomyocytes in a Fibrin Hydrogel for Cardiac Tissue Engineering

by adding 500 U of thrombin to 18 mL of 0.9% saline and 2 mL of sterile deionized water, sterile filter through a 0.2 μm filter, aliquot into 500 μl and 250 μl aliquots and freeze at -80°C.    3)      Prepare a 5% w/v Pluronics F-127 solution

Ex Ovo Electroporation of DNA Vectors into Pre-gastrulation Avian Embryos

as an electrical insulator. Equipment Coverslips (35 mm, No. 1), glass Filter paper rings for embryo removal (see Step 8) Forceps Incubator at 37°C Microcapillaries (1.0-mm O.D. with glass filament) (Narishige, GD-1)