赛默飞世尔Thermo Fisher X8 Filter d
evice Millipore Amicon 0.5mL centrifuga l tubes 100,000 MWCO faster than Microcon: 10 to 货号：T_70310527164- ￥70 - 1400
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- 2025年07月13日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
999
- 供应商：
北京泽平
- 现货状态：
热销品大量现货，其余请咨询
- 保修期：
1年
- 规格：
EA
货号：T_70310527164
中文名称：
英文名称：X8 Filter device Millipore Amicon 0.5mL centrifuga l tubes 100,000 MWCO faster than Microcon: 10 to
货期：热销品现货，其他请咨询
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文献和实验Protocol for Trichloroacetic Acid (TCA) Precipitation
and Equipment Required Borosilicate tubes, 12 x 75 mm (e.g., Fisher Cat #14-961-26) 10% TCA (e.g., Acros Cat # 42145-5000; make a 10% (w/v) solution in water) Warning: TCA is caustic! Carrier nucleic acid, 1 mg/ml (e.g., sheared fish
TOP10 chemically competent cells
10 mM KOAc pH 7.0 (10 ml of a 1M stock/L) 80 mM CaCl2 .2H2 O (11.8 g/L) 20 mM MnCl2 .4H2 O (4.0 g/L) 10 mM MgCl2 .6H2 O (2.0 g/L) 10% glycerol (100 ml/L) adjust pH DOWN to 6.4 with 0.1N HCl if necessary
Purification of recombinant sBRF M166L
if not all the sup. can be removed. Wash the beads 5X with ~35 ml Wash Buffer. After the final wash, transfer the beads to 15ml conical tubes with Wash Buffer. Centrifuge the beads and try to remove all of the supernatant with a Pasteur pipette leaving the beads
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