赛默飞世尔Thermo Fisher X50 Repet-T
ips Gilson(R) REPETMAN syringe type standard polypropylene 25mL (pack of 50) 货号：T_70312609611- ￥70 - 1400
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999
- 供应商：
北京泽平
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- 保修期：
1年
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EA
货号：T_70312609611
中文名称：
英文名称：X50 Repet-Tips Gilson(R) REPETMAN syringe type standard polypropylene 25mL (pack of 50)
货期：热销品现货，其他请咨询
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文献和实验Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
analysis. Restriction enzyme buffer (10X) Dilute the 10X stock to 1.14X for Steps 12 and 13. RNase A, DNase-free (10 mg/mL; Sigma R6513) SDS (Sodium dodecyl sulfate; 20% w/v; Fisher BP166) Sodium acetate (3 M, pH 5.2
PRODUCTION OF ES CELL CHIMERAS BY AGGREGATION WITH EIGHT-CELL STAGE EMBRYOS
will not form gametes, therefore favoring the germ line contribution of ES cells containing the desired mutant allele. This arrangement (XX host morula, XY ES cells) can occur in 50% of the single embryo aggregations, but only in 25% of the sandwich type
Competent cells, calcium chloride method, E. coli, description
. Grow 5 ml overnight of the strain in 2 X YT in a standard container. Screw cap tight and lie flat on the shaker for good aeration. Dilute overnight culture 1/100 - 1/200 to 25, 50 or 100 ml 2 X YT broth in flasks 5 - 10 X culture volume (i.e
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