赛默飞世尔Thermo Fisher X6 Cryobox

ChIP protocol for X. laevis Lens1/FoxE3 enhancer

(9)  Sonicate the homogenate on ice (10 sec. pulse + 50 sec. break x 6 times, 20% amplitude with a small tip of Branson sonifier (Fisher Dismembranator Model 500)). This step releases chromatin DNA from cells without solubilizing yolk proteins that make

Flow Cell Assays with Microtubules: Motility/Dynamics in Fluorescence and VE-DIC

I. Solutions & Supplies BRB80 (1X): 80 mM PIPES, 1 mM MgCl2 , 1 mM EGTA, pH 6.8 with KOH (generally made as a 5X stock and stored at 4¡C) 5 mg/ml casein (Sigma C-5890; dissolve at 10-20 mg/ml in 20 mM Tris, pH 8.0, on a rotator in coldroom

Flow Cell Assays with Microtubules: Motility/Dynamics in Fluorescence and VE-DIC

Assays A. Motility B. Dynamics III. Fluorescence Assays A. Oxygen Scavenging B. Assays Back to protocols I. Solutions & Supplies BRB80 (1X): 80 mM PIPES, 1 mM MgCl2 , 1 mM EGTA, pH 6.8 with KOH (generally made as a 5X