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- 文献和实验
- 技术资料
- 库存:
999
- 供应商:
北京泽平
- 现货状态:
热销品大量现货,其余请咨询
- 保修期:
1年
- 规格:
EA
货号:T_70312502716
中文名称:
英文名称:X2 FIXATIVE CYTORICH RED 500ML RBCs and solubilises proteins 500mL
货期:热销品现货,其他请咨询




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文献和实验4 litres $104 ("Laboratory grade") Sigma 3 kg $42 (no purity details) Acros Organics (seems to be part of Fisher) sell three grades: 2.5 litres $24 (99%) 1 kg $23 (GC) 500 ml $36 (scintillation grade) If the 99% stuff is OK for histology
1) Wash cells twice with PBS and lyse in appropriate lysis buffer; Dish size Wash vol. Lysis vol. 1 well 1ml 125-250μl 6cm plate 3mls 250-500μl 10cm plate 5mls
Single and Double FISH protocols for Drosophila
1. Chlorine bleach; diluted 1:1 with water. 2. 40% formaldehyde solution (prepared fresh from paraformaldehyde as described below). 3. 10X PBS solution. 4. Heptane. 5. Methanol. 6. 20 ml Disposable Glass Scintillation Vials (Fisher).
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