赛默飞世尔Thermo Fisher X72 BOTTLE,

PRODUCTION OF COMPLETELY ES CELL-DERIVED FETUSES BY AGGREGATION WITH TETRAPLOID

    Dissecting microscope;       Acid Tyrode's (e.g. Sigma: T1788);       Sterile plastic Petri dishes (100 x 15 mm);       M2 and KSOM medium in 1 ml syringes;       9'' Pasteur pipettes;       Mouth or Finger controlled pipette. Method: Place

Encapsulation of Cardiomyocytes in a Fibrin Hydrogel for Cardiac Tissue Engineering

with ice in the sterile culture hood.    2)      Place a 250 mL beaker with 30-40 mL of Betadine into the hood.    3)      Add 50 mL/litter of PBS-glucose into a bottle, seal and place into a 37°C water bath.    4)      For each person

PRODUCTION OF ES CELL CHIMERAS BY AGGREGATION WITH EIGHT-CELL STAGE EMBRYOS

Sterile plastic Petri dishes (100 x 15 mm);  M2 and KSOM medium in 1 ml syringes;  9'' Pasteur pipettes;  Mouth or Finger controlled pipette.  Method:  1. Place a few drops of M2, KSOM and acid Tyrode's