赛默飞世尔Thermo Fisher X50 FILTER

Nucleobond Column BAC DNA Purification for Transgenic Mouse Production

. Put the folded filter onto a 50 ml tube or equilibrated Nucleobond AX cartridge, wet it with 0.5 - 1.0 ml of water and fill it with the cooled lysate. Collect the cleared flowthrough. These filters are suitable for plasmid isolation with Nucleobond AX 100

BAC DNA Purification for Transgenic Mouse Production：Microinjection Buffer

7.5 (autoclaved) 0.1 mM EDTA, pH 8.0  10 microliters of 0.5 M EDTA, pH 8.0 (autoclaved) 100 mM NaCl   1 ml of 5 M NaCl (autoclaved) 1x Polyamines   50 microliters 1000x Polyamines mix Autoclaved H2O   up to 50 ml NOTE: Prepare fresh and discard

Alkaline Lysis and CsCl Purification of BAC DNA for Transgenic Production.

, pH 8.0 to 100 ml 6.  Add 20ml of SolutionII per each 250ml of culture, swirl to mix (DO NOT INVERT) and let stand for 5 minutes at room temperature. Solution II (make up fresh EVERY time)  for 50ml 0.2M NaOH       1ml of 10N NaOH (20g