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货号:T_70312940325
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英文名称:200 PAIR Ear plugs 3M(TM) E-A-Rsoft(TM) FX 39dB SNR yellow/green uncorded (pack of 200 x 1pair)
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文献和实验. Resuspend the DNA in 10:0.1 TE buffer. E. Single-stranded M13 DNA isolation using phenol This isolation procedure (23) is the method of choice for preparation of M13-based templates to be used in Sequenase[TM] catalyzed dye
Bisulfite sequencing of very small samples
can be also used for analyzing up to 200ng purified genomic DNA in one sample Back to top Procedure Primer design Good primers are absolutely critical for successful bisulfite sequencing. Use genomic DNA sequence from the region you want
PCR PRIMER DESIGN AND REACTION OPTIMISATION
of the Tm is Tm = 4(G + C) + 2(A + T)o C. Thus, the annealing temperature chosen for a PCR depends directly on length and composition of the primer(s). One should aim at using an annealing temperature (Ta) about 5o C below the lowest Tm of ther pair
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