赛默飞世尔Thermo Fisher X1000 Pipet

基本实验技术

found in the refrigerators, incubators, or freezers may be destroyed. Always mark the backs of the plates with your initials, the date, and relevant experimental data, e.g. strain numbers. II. PREPARATION OF SOLUTIONS A. Calculation of Molar, % and "X

【精华】vol 687 Chapter 3 采用Splinkerette-PCR技术对前病毒基因组插入位点进行分离

is the same as that in STA-PCR. Fig. 1. Schematic of strategies for cloning genomic DNA sequence flanking a proviral insertion. (a) STA-PCR protocol. Genomic DNA (horizontal lines) is digested with a cocktail of restriction enzymes (noted as E, F, and G

In Situ HYBRIDIZATION TO TISSUE SECTIONS

. The devittellinized embryos will sink to the bottom. Transfer the embryos to a vial with a wide bore pipet tip. Wash 3X with 90% MeOH/EGTA. Rehydrate embryos (0-2 and 1-3 hr embryos need not be rehydrated through paraformaldehyde, just leave