赛默飞世尔Thermo Fisher 450 MM Tubi

【精华】vol 687 Chapter 3 采用Splinkerette-PCR技术对前病毒基因组插入位点进行分离

of an adenosine, by taking advantage of the terminal nontemplatedextension capacity of conventional Taq DNA polymerases(31, 32). This Addition step is followed by ligation toSplinkTA, a modified splinkerette linker, which is made up ofSplinkerette (the hairpin

Measurement of Nitric Oxide in Single Cells and Tissue Using a Porphyrinic Microsensor

micromanipulators, resolution 1 µm or better Platinum wire (platinum auxiliary or counter electrode; 0.5‐ to 1‐mm diameter, 3‐cm length)

Stripping Western Blots

. 9) Wash twice with cold wash buffer (0.1 M NaCl, 0.25 M Tris-HCl, pH 7.5.). 10) To washed immunocomplex, add 40 μl of reaction mixture (10 mM Tris-HCl, pH 7.5, 10 mM MgCl2, 1 mM DTT, 25 μM ATP, 5 μCi 32P-ATP (Dupont NEN Cat# NEG-002A) and 10 μg