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- 详细信息
- 文献和实验
- 技术资料
- 库存:
999
- 供应商:
北京泽平
- 现货状态:
现货或咨询
- 保修期:
不适用
- 规格:
请咨询
货号:3472ANK
中文名称:0.5ml,无凸纹,可立,棕色,非灭菌,含盖,管
英文名称:0.5ml Screw Cap Tube, NonKnurl,Skirted,Amber, Non-Sterile tube w/ unattached cap
品牌:Thermo Fisher 赛默飞世尔
货期:热销品现货,其他请咨询






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文献和实验Materials2 ml size Sarstedt screw cap tubesBIO-RAD Disposable Chromatography Column (BIO-RAD cat. #732-6008)Oligo dT celluloseRNase-free water5.0 M NaCl0.5 M EDTA1.0 M Tris, pH 7.410% SDS70% EtOHBefore starting protocol1. Measure out 75 mg of oligo
Phosphoamino acid analysis:Mark Kamps's method
will work! Keep the membrane wet and wrap in Saran wrap. Add radioactive markings for orientation of filmlater Expose to film. 2.Cut out band of interest,re-wet in methanol,rinse well in water and place in a screw-cap tube containing 150 µl 5.7 NHCl
Phosphoamino acid analysis:Mark Kamps method
in a screw-cap tube containing 150 µl 5.7 N HCl or enough to submerge your piece of membrane. 3. Incubate in 110°C oven for 60 min. 4. Microfuge sample full speed for at least 1 min. 5. Transfer supernatant to new tube and lyophilize
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