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- 技术资料
- 库存：
999
- 供应商：
北京泽平
- 现货状态：
热销品大量现货，其余请咨询
- 保修期：
1年
- 规格：
EA
货号：AB-0661
中文名称：
英文名称：ABgene 2.2 ml Storage Plate (Individually wrapped)
货期：热销品现货，其他请咨询
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文献和实验General Laboratory Procedures, Equipment Use, and Safety Considerations
or from a previously-tested glycerol stock that was grown from a single colony. To prepare an overnight culture from a glycerol stock, take an individually-wrapped 1-ml pipet and a culture tube of media to the -80 deg C freezer. Quickly remove the cap from the freezer
Large-scale yeast transformation
. Discard water and resuspend cells by vortexing in residue. We then add 140 ul of water, mix by pipetting, and plate 6 ul to SC-leu -ura plate. We use the rest of the suspension for filter assays and -70o C storage. PEG: 400 mls 50% PEG
Large-scale yeast transformation大规模转化酵母【Yale University】
once with water. Discard water and resuspend cells by vortexing in residue. We then add 140 ul of water, mix by pipetting, and plate 6 ul to SC-leu -ura plate. We use the rest of the suspension for filter assays and -70℃ storage. 1.PEG: 400 mls 50% PEG, 50 mls
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