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- 技术资料
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999
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北京泽平
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- 保修期：
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货号：263
中文名称：
英文名称：Samco 23.0mL Extra Long Transfer Pipets, 12", Non-sterile
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文献和实验, ending with an extensive wash (25ml) in TTBS, containing an extra 0.2 M NaCl. The column is now ready for use. For long term storage, cap bottom of column and wrap Parafilm around the top and store at 4°C (there is azide in the TTBS). Wash Buffer
Non-fluorescent protein/RNA double-labeling
to the bottom) and wash the embryos well with methanol. Transfer the embryos to a 0.5 ml eppendorf tube. 2. Wash the embryos with 3% hydrogen peroxide in methanol for 15 min. Rinse well in methanol. 3. Wash 3 X 30 minutes with PBTH (filtered 1 X PBS, 0.1% Tween-20
Non-fluorescent protein/RNA double-labeling
is 5% formaldehyde, 0.05M EGTA in 1X PBS. pH 7.0. Devitellinize embryos by shaking in an equal mixture of methanol:heptane (embryos will sink to the bottom) and wash the embryos well with methanol. Transfer the embryos to a 0.5 ml eppendorf tube. 2. Wash
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