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文献和实验Large-scale yeast transformation
. We omitted this for months. It didn't seem to affect transformation efficiency at all, but maybe it affects non-homologous integration? Large Scale Transformation Dispense yeast competent cells into deepwells with DNA (100 ul/well).
Large-scale yeast transformation大规模转化酵母【Yale University】
-homologous integration? Dispense yeast competent cells into deepwells with DNA (100 ul/well). Capmat deepwells. Incubate at 30℃, 30 mins, on side. Resuspend cells by vortexing. Dispense PEG 1 into deepwell wih yeast/DNA (250 ul/well). Pipette
PRODUCTION OF COMPLETELY ES CELL-DERIVED FETUSES BY AGGREGATION WITH TETRAPLOID
tip) alternatively: Finger controlled pipette (Manostat tubing, yellow tip, scalpel blade); 9'' Pasteur pipettes; 70% Ethanol; Alcohol burner or Bunsen burner; Sterile plastic Petri dishes (100 x 15 mm); Sterile
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