Sartorius赛多利斯S7594------FMOSKM

Screening lambda cDNA or genomic libraries

need 6.6 ml top agarose (use a bigger tube and a 50-55° water bath).to titer (on 100 mm plates):Put various dilutions of phage in 100 µl 1X phage buffer.Always do a no phage control! This will help you distinguish between dilutions that give you no phage (look

DNA疫苗实验方法

you want). See "Plasmid DNA Preparation" protocols for detailed information. Each TA muscle is injected with 50 µl of DNA solution. To inject plasmid DNA use a 27GX3/4" (0.4x20mm) needle attached to a 1 ml tuberculin syringe. A piece of polyethylene

Construction and Characterization of Adenovirus Vectors

stock solution with H2 O and sterilize by autoclaving for 45 min at 121°C. Complete medium Dialysis buffer (10 mM Tris-HCl, pH 8.0) Sterilize by filtration through a 0.2-µm filter. DNase I (Ad) Ethidium bromide