- ￥6470 - 47940
- Sino Biological
- 北京
- 12504-H82H
- 2026年01月26日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃ to -80℃
- 保质期：
12个月
- 英文名：
Recombinant Human CD19 Protein (Site-Specific AF 647 Conjugation, ECD, His Tag)
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 规格：
20.00 µg/100.00 µg/500.00 µg
| 规格： | 20.00 µg | 产品价格： | ￥6470.0 |
|---|---|---|---|
| 规格： | 100.00 µg | 产品价格： | ￥13620.0 |
| 规格： | 500.00 µg | 产品价格： | ￥47940.0 |
蛋白名称：Human CD19 Protein (Site-Specific AF 647 Conjugation, ECD, His Tag)
蛋白构建：A DNA sequence encoding the extracellular domain (Met1-Lys291) of human CD19 (P15391-1) was expressed with a C-terminal polyhistidine tag. The protein is site-specifically conjugated with AF 647 (Excitation Max.= 655 nm, Emission Max.= 680 nm).
蛋白纯度：
蛋白活性：1.Flow cytometric analysis of anti-CD19 CAR expression. 293 cells were lentivirally transduced with anti-CD19 CAR. Flow cytometric analysis was performed with a negative control protein and AF 647-conjugated human CD19 protein (Site-Specific Conjugation, ECD, His Tag, Cat. No. 11880-H08W1-SG), respectively. Non-transduced 293 cells were used as a control (left). 2.Binding activity of AF 647-conjugated CD19 protein to PBMC cells. PBMC cells were stained with anti-CD3 antibody and AF 647-conjugated human CD19 protein (Site-Specific Conjugation, ECD, His Tag, Cat. No. 11880-H08W1-SG) and detected by flow cytometry. PBMC cells stained with anti-CD3 antibody were used as a control (left). 3.Loaded Biotinylated Inebilizumab on SA Biosensor, can bind Recombinant Human CD19 Protein (Site-Specific AF 647-Conjugated, ECD), His Tag (Cat. No. 11880-H08W1-SG) with an affinity constant of 0.651 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested).
蛋白内毒素：< 1.0 EU per μg protein as determined by the LAL method.
预测N端：Pro 20
蛋白分子量：The protein has a predicted molecular mass of 31.6 kDa.
蛋白NP号：P15391-1
蛋白氨基酸序列：Met1-Lys291
蛋白标签：C-His
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文献和实验、CD14-PE、CD19-PE、CD3-PE、CD66b-PE、CD56-PE-Cy7、CD11c-APC、 CD45-AF700、CD38-APC-AF750（每次测试 5μL），以及 CD16-FITC（每次测试 20 μL），并在冰上孵育 30 分钟。10. 在 4℃ 条件下，300 x G 离心细胞 5 分钟。11. 吸取上清液。12. 重悬到 500 μL 染色缓冲液中。13. 通过 CytoFLEX 获取结果。通过 CytoFLEX 进行数据采集：1. 创建新补偿值。2. 如适用
Generation of Dendritic Cells from Lymphoid Precursors
. Methods have become available to grow DC in culture, facilitating the study of their biological and developmental properties. One such method is described in this chapter to generate DC in vitro from CD34+ Lineage- (Lin- ) CD10+ bone marrow (BM) progenitor
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