- ￥6470 - 47940
- Sino Biological
- 北京
- 12504-H82H
- 2025年08月12日
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- 详细信息
- 技术资料
- 保存条件：
-20℃ to -80℃
- 保质期：
12个月
- 英文名：
Recombinant Human CD19 Protein (Site-Specific AF 647 Conjugation, ECD, His Tag)
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 规格：
20.00 µg/100.00 µg/500.00 µg
| 规格： | 20.00 µg | 产品价格： | ￥6470.0 |
|---|---|---|---|
| 规格： | 100.00 µg | 产品价格： | ￥13620.0 |
| 规格： | 500.00 µg | 产品价格： | ￥47940.0 |
CD19重组蛋白|Recombinant Human CD19 Protein (Site-Specific AF 647 Conjugation, ECD, His Tag)（产品说明）
蛋白名称：Human CD19 Protein (Site-Specific AF 647 Conjugation, ECD, His Tag)
蛋白构建：A DNA sequence encoding the extracellular domain (Met1-Lys291) of human CD19 (P15391-1) was expressed with a C-terminal polyhistidine tag. The protein is site-specifically conjugated with AF 647 (Excitation Max.= 655 nm, Emission Max.= 680 nm).
蛋白纯度：
蛋白活性：1.Flow cytometric analysis of anti-CD19 CAR expression. 293 cells were lentivirally transduced with anti-CD19 CAR. Flow cytometric analysis was performed with a negative control protein and AF 647-conjugated human CD19 protein (Site-Specific Conjugation, ECD, His Tag, Cat. No. 11880-H08W1-SG), respectively. Non-transduced 293 cells were used as a control (left). 2.Binding activity of AF 647-conjugated CD19 protein to PBMC cells. PBMC cells were stained with anti-CD3 antibody and AF 647-conjugated human CD19 protein (Site-Specific Conjugation, ECD, His Tag, Cat. No. 11880-H08W1-SG) and detected by flow cytometry. PBMC cells stained with anti-CD3 antibody were used as a control (left). 3.Loaded Biotinylated Inebilizumab on SA Biosensor, can bind Recombinant Human CD19 Protein (Site-Specific AF 647-Conjugated, ECD), His Tag (Cat. No. 11880-H08W1-SG) with an affinity constant of 0.651 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested).
蛋白内毒素：< 1.0 EU per μg protein as determined by the LAL method.
预测N端：Pro 20
蛋白分子量：The protein has a predicted molecular mass of 31.6 kDa.
蛋白NP号：P15391-1
蛋白氨基酸序列：Met1-Lys291
蛋白标签：C-His
蛋白名称：Human CD19 Protein (Site-Specific AF 647 Conjugation, ECD, His Tag)
蛋白构建：A DNA sequence encoding the extracellular domain (Met1-Lys291) of human CD19 (P15391-1) was expressed with a C-terminal polyhistidine tag. The protein is site-specifically conjugated with AF 647 (Excitation Max.= 655 nm, Emission Max.= 680 nm).
蛋白纯度：
蛋白活性：1.Flow cytometric analysis of anti-CD19 CAR expression. 293 cells were lentivirally transduced with anti-CD19 CAR. Flow cytometric analysis was performed with a negative control protein and AF 647-conjugated human CD19 protein (Site-Specific Conjugation, ECD, His Tag, Cat. No. 11880-H08W1-SG), respectively. Non-transduced 293 cells were used as a control (left). 2.Binding activity of AF 647-conjugated CD19 protein to PBMC cells. PBMC cells were stained with anti-CD3 antibody and AF 647-conjugated human CD19 protein (Site-Specific Conjugation, ECD, His Tag, Cat. No. 11880-H08W1-SG) and detected by flow cytometry. PBMC cells stained with anti-CD3 antibody were used as a control (left). 3.Loaded Biotinylated Inebilizumab on SA Biosensor, can bind Recombinant Human CD19 Protein (Site-Specific AF 647-Conjugated, ECD), His Tag (Cat. No. 11880-H08W1-SG) with an affinity constant of 0.651 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested).
蛋白内毒素：< 1.0 EU per μg protein as determined by the LAL method.
预测N端：Pro 20
蛋白分子量：The protein has a predicted molecular mass of 31.6 kDa.
蛋白NP号：P15391-1
蛋白氨基酸序列：Met1-Lys291
蛋白标签：C-His
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CD19重组蛋白|Recombinant Human CD19 Protein (Site-Specific AF 647 Conjugation, ECD, His Tag)
￥6470 - 47940
