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- 文献和实验
- 技术资料
- 保存条件:
-20℃ to -80℃
- 保质期:
12个月
- 英文名:
CDK9/CyclinT1, Active
- 库存:
99
- 供应商:
北京义翘神州科技股份有限公司
- 规格:
10 µg
蛋白名称:CDK9/CyclinT1, Active
蛋白构建:Recombinant full length human CDK9 and CyclinT1 proteins were co-expressed by baculovirus in Sf9 insect cells using a N-terminal GST tag.
表达宿主:Baculovirus-Insect Cells
蛋白分子量:CDK9 ~68 kDa; CyclinT1 ~110 kDa
蛋白NP号:NM_001261 ,NM_001240
蛋白氨基酸序列:Full Length
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文献和实验Two-hybrid analysis of genetic
fused protein (prey)is mated with different yeast strains which express either the original bait protein or other,preferably unrelated baits,and the investigator verifies that the reporters are only active in diploids that contain the original bait
-3b, sharing97% sequence similarity within their kinase catalytic domains(2) . In contrast to most kinases, GSK-3 is inactivated by phosphorylation(2) . GSK-3 is active in nonstimulated cells, whereasother kinases require cell stimulation for activation
Two-hybrid analysis of genetic regulatory networks
the activation domain fused protein (prey) is mated with different yeast strains which express either the original bait protein or other, preferably unrelated baits, and the investigator verifies that the reporters are only active in diploids that contain
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