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- 详细信息
- 文献和实验
- 技术资料
- 抗体名:
HRP-conjugated beta actin Monoclonal Antibody_货号:AN00295HP_标签内参抗体
- 抗体英文名:
HRP-conjugated beta actin Monoclonal Antibody_货号:AN00295HP_标签内参抗体
- 应用范围:
WB
- 宿主:
Mouse
- 适应物种:
Human;Mouse;Rat
- 保质期:
12个月
- 目录编号:
AN00295HP
- 级别:
科研级别
- 库存:
999
- 供应商:
武汉伊莱瑞特生物科技股份有限公司
- 标记物:
HRP
- 克隆性:
Monoclonal
- 保存条件:
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. Protected from prolonged exposure to light.
- 形态:
液体
- 亚型:
IgG1
- 规格:
100μL/25μL
| 规格: | 100μL | 产品价格: | ¥920.0 |
|---|---|---|---|
| 规格: | 25μL | 产品价格: | ¥350.0 |
产品名称:HRP-conjugated beta actin Monoclonal Antibody
货号:AN00295HP
| Verified Samples |
Verified Samples in WB:Raji,Jurkat,HL-60,U87-MG,Mouse lung,Rat lung,Raw264.7,C6
|
| Dilution |
WB 1:2500-1:5000
|
| Clonality |
Monoclonal
|
| Isotype |
IgG1
|
| Immunogen |
Recombinant human Beta actin protein by E. Coli
|
| Abbre |
beta actin
|
| Synonyms |
ACTB;Actin beta;Actx;Actin cytoplasmic 1;b actin;A26C1A
|
| Swissprot |
P60709
|
| Calculated MW |
43kDa
|
| Observed MW |
43kDa
|
| Cellular Localization |
Cytoplasm>cytoskeleton. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
|
| Concentration |
1mg/mL
|
| Storage |
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. Protected from prolonged exposure to light.
|
| Buffer |
PBS with 0.1% procline 300,1% protective protein and 50% glycerol,pH7.4
|
| Purification Method |
Protein A/G Purification
|
| Research Areas |
Signal Transduction;Tags and Cell Markers;Isotype/Loading;Controls;Neuroscience;Biochemicals;Cancer;Cardiovascular;Cell Biology;Immunology;Metabolism;Epigenetics and Nuclear Signaling;Stem Cells
|
| Clone No. |
Da-3A9
|
| Conjugation |
HRP
|
| Background |
Beta-actin (ACTB/ACTB) is one of six different actin isoforms in humans. Actins are highly conserved proteins that are involved in cell motility,structure and integrity. Beta actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. Beta-actin gene is stably and constitutively expressed at high levels in most tissues and cells,and commonly used as a loading control for western blot and as a control for qPCR.
|

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文献和实验Western Blotting with Monoclonal Ant
the blocking buffer from the blot, add the antibody solution, and incubate with agitation for 30 minutes at 37˚C, one hour at room temperature, or overnight at 4˚C. Enzyme conjugated secondary antibody Note: The inclusion of sodium azide is to be avoided
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Western Blotting with Monoclonal Antibodies
the antibody in the corresponding blocking buffer. Decant the blocking buffer from the blot, add the antibody solution, and incubate with agitation for 30 minutes at 37˚C, one hour at room temperature, or overnight at 4˚C. Enzyme conjugated
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