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- 2025年12月05日
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| 规格： | 10 mg | 产品价格： | ￥208.0 |
|---|---|---|---|
| 规格： | 25 mg | 产品价格： | ￥384.0 |
| 规格： | 50 mg | 产品价格： | ￥584.0 |
| 规格： | 100 mg | 产品价格： | ￥880.0 |
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文献和实验of mRNA (poly-A+) from total RNA using oligo-dT-affinity also reduces DNA contamination. One can also add to the RNA sample a combination of restriction enzymes to specifically cleave genomic DNA. Ashkenas et al. (Biotechniques 39:1 2005) suggest
Real-Time qRT-PCR standard curves... efficiency is too h
) PCR should not give you any band. If it does, treat you standard again with DNase I. Note: when you do a cloning please in clude 10-20 bp extra flanking at the both 3' and 5' end of your acture mRNA target. This will provide bater
Quantitation of CD39 Gene Expression in Pancreatic Tissue by Real-Time Polymerase Chain Reaction
real-time (qRT) polymerase chain reaction (PCR), and microarrays. Although every technique has its specific application, the high levels of accuracy, reproducibility, sensitivity, and specificity have established qRT-PCR as a standard method
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