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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Please store the product under the recommended conditions in the Certificate of Analysis.
- 库存:
货期:1-2天
- 供应商:
MedChemExpress LLC
- CAS号:
94-20-2
- 规格:
25 mg/50 mg/100 mg/250 mg
| 规格: | 25 mg | 产品价格: | ¥350.0 |
|---|---|---|---|
| 规格: | 50 mg | 产品价格: | ¥530.0 |
| 规格: | 100 mg | 产品价格: | ¥800.0 |
| 规格: | 250 mg | 产品价格: | ¥1500.0 |
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文献和实验START" PCR.NOTE:USE PLUGGED PIPETTE TIPS: prevents aerosol contamination of pipettes.Use of detergents is recommended only for Taq from Promega (up to 0.1% v/v, Triton X-100 or Tween-20). DMSO apparently allows better denaturation of longer target
contamination will be of larger size or may even not be obtained (if the introns are large). Step II - PCR A fraction, usually 5-20%, of the RT products is directly used in PCR using gene-specific primers. Notes Genomic DNA contamination
Thermal Cycling Profile for Standard PCR
Initial denaturation It is very important to denature the template DNA completely. Initial heating of the PCR mixture for 2 minutes at 94°�95°C is enough to completely denature complex
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