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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Please store the product under the recommended conditions in the Certificate of Analysis.
- 英文名:
Ro 31-8959 (Standard)
- 库存:
货期:询盘
- 供应商:
MedChemExpress LLC
- CAS号:
127779-20-8
- 规格:
5 mg/10 mg/25 mg/50 mg/100 mg
| 规格: | 5 mg | 产品价格: | ¥945.0 |
|---|---|---|---|
| 规格: | 10 mg | 产品价格: | ¥1512.0 |
| 规格: | 25 mg | 产品价格: | ¥3024.0 |
| 规格: | 50 mg | 产品价格: | ¥4842.0 |
| 规格: | 100 mg | 产品价格: | ¥7263.0 |
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文献和实验START" PCR.NOTE:USE PLUGGED PIPETTE TIPS: prevents aerosol contamination of pipettes.Use of detergents is recommended only for Taq from Promega (up to 0.1% v/v, Triton X-100 or Tween-20). DMSO apparently allows better denaturation of longer target
Stewart Laboratory Standard Operating Procedure
x g, 1700 rpm, 5 min in 15 ml tubes - no faster!), and resuspend in 10 mL 70% cold EtOH. Store at -20°C overnight or for several days Day 2 Pellet cells and wash once with wash buffer (PBS + 0.5% IFS). Pellet and resuspend in 0.5 mL 2M HCl
contamination will be of larger size or may even not be obtained (if the introns are large). Step II - PCR A fraction, usually 5-20%, of the RT products is directly used in PCR using gene-specific primers. Notes Genomic DNA contamination
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