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- 文献和实验
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- 保存条件:
4°C, protect from light
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货期:1-2天
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MedChemExpress LLC
- CAS号:
1022-79-3
- 规格:
1 g
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文献和实验Single Primer ("Semi-Random") P
to about 1 ng/µL 3 µL "T/TS" @ 0.4 u Taq/µL -------- 30 µL Cycle as follows: 30"x94°C 20 cycles 0"x94°C 0"x55°C 1'x72° S=9 30 cycles 0"x94°C 0"x40°C 1'x72° S=6 30 cycles 0"x94°C 0"x55°C 1'x72° S=9 Clean up: Add 1 µL ExoI
96-well RNA In Situ Hybridization Protocol
). To stop development of individual wells, remove staining solution and add 200ul PBT. 26. To stop entire plate, rinse plate 3X with PBT. 27. Add 200ul 70% gycerol to each well. 28. Embryos are ready to mount or dissect once they have settled
Hints and precautions for the care, feeding and breeding of Neurospora
Neurospora. Others may be practices unique to our lab. The attempt here is to supplement compilations of methods such as Davis and de Serres (1970 Methods in Enzymology 17A:79-143) and the older Stanford Neurospora Methods (1963 Neurospora Newsletter 4:21-25
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