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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Please store the product under the recommended conditions in the Certificate of Analysis.
- 库存:
货期:询盘
- 供应商:
MedChemExpress LLC
- 规格:
1 KU
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文献和实验Pronase is the name given to a group of proteolytic enzymes that are produced in the culture supernatant of Streptomyces griseus K-1 (1 –3 ). Pronase is known to contain at least ten proteolytic components: five serine-type proteases, two Zn
【信息】Northstar BioProducts(NSBP)
100174-1 Alkaline Protease, (Streptomyces grisues), EC 3.4.21.14 50 mg Microbial serine proteinase. Lyophilized powder. Activity: 20 units/mg protein M.W.: ~50,000 (SDS-PAGE) 100308-1 Cholesterol Esterase, (Candida cylindracea
8.0),-20度保存1小时后,13000rpm离心15min,弃上清液,沉淀用70%乙醇洗2次;置于室温干燥后,溶于50ulTE溶液中,置4oC保存备用。 2 蛋白酶/SDS法制备 先用10ml含适当抗生素的GBM过夜培养Delftia sp.,第二天4000rpm离心10min收集菌体,用Washing TE(50mmol/LTris-HCl pH8.0,10mmol/LEDTA pH8.0)洗菌体2次,之后将菌体充分悬浮在5ml 1×TE缓冲液中,先后加入0.5ml 5mg/L的蛋白酶
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