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- 英文名:
BSA PMMA Particles
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100
- 供应商:
百欧泰(biotyscience)
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文献和实验MINICHROMOSOME MICROTUBULE BINDING ASSAY
Determine the OD600 and correlate the cell density from the chart. Set up four 100mL YPD cultures at the following densities: 0.7x105 , 1x105 , and 3.0x105 cells/mL GROWTH OF CELLS Grow 100mL of cells to OD600 =0.7-0.8 at 23o C
Minichromosome-Microtubule Binding Assay 微染色体-微管结合实验
http://www.ciwemb.edu/labs/koshland/Protocols/MICROTUBULE/mmb.html Determine the OD600 and correlate the cell density from the chart. Set up four 100mL YPD cultures at the following densities: 0.7x105 , 1x105 , and 3.0x105 cells/mL
微染色体-微管结合实验(Minichromosome-Microtubule Binding Assay)
(containing 0.1mM DTT, 100mM NaCl, 5% glycerol and 1mg/ml BSA, but NO PMSF!). These are the PELLET FRACTIONS. NOTE: Ultimately, PELLETs will be 2x concentrated relative to SUPs. Add 250ul of 2x ASSAULT buffer (containing tRNA and øX174
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