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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
Protein G PDLA Particles
- 库存:
100
- 供应商:
百欧泰(biotyscience)
- 规格:
10 mg/ml10ml
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文献和实验GST Fusion Protein Purification from Yeast
Keep samples cold between vortexing. Poke a hole in the bottom of the tube and spin cell lysate in a new microfuge tube 1.5 K or 500 X g, 10 min., 4o C. Transfer liquid from bottom tube into a new microfuge tube. Spin again 1.5 K
50 mM Tris-HCl, pH 7.6 0.5 ml 1 M Tris-HCl, pH 7.6 1 mM Na2EDTA, pH 8.0 0.1 ml 0.1 M Na2EDTA, pH 8.0 9.6 ml ddH2O 10 ml 4. Fluorescent Labeled Primers Prepare a 100 X stock solution (40 uM); an example
酵母GST蛋白纯化方法 GST Fusion Protein Purification from Yeast
Keep samples cold between vortexing. Poke a hole in the bottom of the tube and spin cell lysate in a new microfuge tube 1.5 K or 500 X g, 10 min., 4o C. Transfer liquid from bottom tube into a new microfuge tube. Spin again 1.5 K
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