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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
Protein A PDLA Particles
- 库存:
100
- 供应商:
百欧泰(biotyscience)
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文献和实验7.8 or Binding Buffer.(6) BD tubing 14-170-12F (Fisher).(7) Polyethylene tubing Clay Adanes.(8) 18 Gauge needles.(9) Binding Buffer (BioRad): make 1L. 314g/L ddH2O and filter through 0.22 um, filter. pH=9.0.(10) Elution buffer (BioRad): make 500 ml. 11g/500 ml
Falcon tubes to determine volume. Dilute the mixture 1:1 with Binding buffer . Filter through 0.45 um filters (use prefilters). For Purification of Abs by Protein A column: Prepare Protein A column. Run binding
and additions: 1.To make saturated culture combine 5 ml of YPD with 25µl of 0.4% adenine and 1 blob of yeast.Incubate at 30℃ for 18 to 24 hr until yeast is at a density of 2.0 x108 cells/ml. 2.Grow cells to an OD600 of ~1.0 after inoculating 1 liter of YPD
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