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- 英文名:
DVB Particles
- 库存:
100
- 供应商:
百欧泰(biotyscience)
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文献和实验Biotin labeling and hybridization of probe to chromosomes
quality) at 70 C for 2 min. Immediately transfer the slides into an ethanol series (70 %, 95 %, and 100 %, 5 min each at 20 C), and air dry the slides. Add 10 µl of the hybridization mixture to each slide and cover with an '18 x 18' mm
Preparation and purification of biotin-labeled probe for ISH
is collected in the 1.5 ml tube The final volume of probe should be close to 55 µl. Usually, 5 µl of such a biotin-labeled probe (1 µg/55 µl = 18 ng/µl) is enough for one slide, and the probe can be used to process 100 slides. The size of the probe
the 3 hour incubation Dynabeads™ M-280 Streptaviden magnetic beads (7x107 beads, ~100µl/sample) are added to the respective samples (note 1);5.The resultant siRNA/Flag complexes are then eluted with magnetic bead binding, the solution can be discarded
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