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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
Protein A DVB Particles
- 库存:
100
- 供应商:
百欧泰(biotyscience)
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文献和实验GST Fusion Protein Purification from Yeast
8, and mix with 6X SDS-PAGE sample buffer before stripping the beads with SDS-PAGE sample buffer. Heat to 100o C for 10 min. Then store at -20o C. Protein is ready to be run on SDS-PAGE Gel. Lysis Buffer (20 ml
酵母GST蛋白纯化方法 GST Fusion Protein Purification from Yeast
8, and mix with 6X SDS-PAGE sample buffer before stripping the beads with SDS-PAGE sample buffer. Heat to 100o C for 10 min. Then store at -20o C. Protein is ready to be run on SDS-PAGE Gel. Lysis Buffer (20 ml
) (1/18 mer)(ul/40 pmoles)=x ul In this example x=160 ul, and 160 ul of ddH20 should be added to the dried tube of fluorescent primer for a concentration of 40 uM (40 pmol/ul). From this 100X stock of 40 uM make 1:100 dilutions. To make
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