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- 文献和实验
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- 英文名:
PVC Particles
- 库存:
100
- 供应商:
百欧泰(biotyscience)
- 规格:
10 mg/ml10ml
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文献和实验Biotin labeling and hybridization of probe to chromosomes
a slide holder that can handle 10-20 slides) in 70 % formamide in 2 X SSC (140 ml formamide + 20 ml 20 X SSC + 40 ml H2O, this solution can be used several times; the quality of the formamide is important, i. e., formamide from CMS is of constant good
3.75ml 4M NaCl 3ml 10% Triton X (Tx-100) 300µl 10% NAD 21.45ml H20 ChIP wash buffer 30ml final volume: 150µl 2M Tris 1.7ml 4.4M LiCl 60µl 0.5M EDTA 1.5ml 10% NAD 1.5ml 10% NP-40 25.09ml
genomic regions in the nucleus in human cell cultures we designed several RNA-biotin based pulldown assays which can be used alone or in combination with other known assays such as ChIP and Flag-tagged pulldown assays. Three protocols are explained
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