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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
PVC Particles
- 库存:
100
- 供应商:
百欧泰(biotyscience)
- 规格:
10 mg/ml10ml
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文献和实验3X Flag Peptide) to each pelleted bead/complex, incubate 30-60 minutes at 4°C with gentle shaking;Centrifuge the resin for 30 seconds at 10,000 RPM, transfer the supernatants (which should now contain the ChIP/Flag eluted complexes) to fresh tubes
CGH of Biotin Labeled DNA vs Digoxigenin Labeled DNA
volume of 3M Na Acetate, mixing with pipet. Add 2.5 X (original) vol 100% EtOH to ppt DNA, vortex gently. Spin 30 mins at 14K rpm, 4 C. Decant supernatant; blot dry, being careful to avoid DNA pellet. Add 10 ul of MM1/H20 mix (70% MM1/30
specific transcripts (pRNAs) with the biotin linked pull-down assay (Figure 2) (comment 5);From the 200µl elute 30µl should be saved as input and the remaining 170µl from the extracted genomic DNA (Qiagen™ DNeasy) is mixed with 170µl of modified lysis
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