Protein G PVC Particles/PVC微球,

Detection and Analysis of Protein‐Protein Interactions of Organellar and Prokaryotic Proteomes by Blue Native and Colorless Native Gel Electrophoresis

1.5‐mm spacers Peristaltic pump and 0.110‐in. i.d., 2.79‐mm PVC tubing 19‐G needles (8‐in. long; 1.00 × 200–mm) with Luer‐connection (e.g., Rometsch GmbH or Popper & Sons) Gradient maker with two chambers

GST Fusion Protein Purification from Yeast

Keep samples cold between vortexing. Poke a hole in the bottom of the tube and spin cell lysate in a new microfuge tube 1.5 K or 500 X g, 10 min., 4o C.  Transfer liquid from bottom tube into a new microfuge tube.   Spin again 1.5 K

PCR基因芯片上荧光PCR反应的研究

流道，又分为很多次流道，每个微反应室的入口与出口就与次通道相连，主流道宽140um，次流道宽50um。微反应室与流道腔的深度分别达到200um与50um。芯片进行热氧化后表面的亲水性增强，反应液可以在芯片内顺利流动。PVC聚合物压合封闭芯片，因为PCR芯片需要事先在其表面点入若干DNA样本或引物，封闭材料不仅有良好的导热性、密闭性、对PCR反应无抑制且在封闭过程中不能对点入的DNA样本有损。 2.2　PCR-SSCP并测序分析发现X连锁遗传性铁幼粒细胞贫血家系ALAS2基因第5外显子基因异常　　分析两