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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
Protein G PVA Particles
- 库存:
100
- 供应商:
百欧泰(biotyscience)
- 规格:
10 mg/ml10ml
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文献和实验Protein Microarrays for Identification of Novel Extracellular Protein‐Protein Interactions
) PBS (see recipe ) Bait protein: can be any extracellular protein of interest to the investigator, e.g., an orphan receptor that functions in cellular communication
) of 125-I using microsyringe 3. Add 1-2 pieces of IODO-BEADs¶ 4. incubate at room temp, 5 min 5. Add your protein(1-100µg, 25 µg is good for IgGs or Fab) 6. Incubateat room temp, 10-25 min 7. Take reaction mixture (leave beads) and apply
A. Method for ~1g or more of tissue. 1. Label all tubes. Prepare solutions and have ready at hand. 2. Remove the tissue from the �80oC freezer and thaw on ice. If the tissue is fresh, keep on ice (or alternatively work in a cold room).
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