- 询价
- 百欧泰(biotyscience)
- 北京
- ABPET-7000-Protein A
- 2025年07月06日
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- 详细信息
- 文献和实验
- 技术资料
- 英文名：
Protein A PET Particles
- 库存：
100
- 供应商：
百欧泰(biotyscience)
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文献和实验Identifying Protein Interactions by Hydroxyl‐Radical Protein Footprinting
test protein's open reading frame Modified vectors (available from Nick Loizos on request) of the pET expression system:
1 to 4 of protocol 1 , but omit IPTG in step 4) Cells expressing protein fusions and an untagged RNA template (pET plasmid, Novagen)
Purification of TFIIA from E. coli
,quickly frozen and stored at -70 degrees. Cells are thawed,resuspended in 60ml RB Buffer+ 5mM DTT+ protease inhibitors and disrupted by two passes through the microfluidizer.Insoluble material is pelleted for 10 minutes at 8.5K in GSA rotor at 4 degrees
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