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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
PET Particles
- 库存:
100
- 供应商:
百欧泰(biotyscience)
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文献和实验Biotin labeling and hybridization of probe to chromosomes
a slide holder that can handle 10-20 slides) in 70 % formamide in 2 X SSC (140 ml formamide + 20 ml 20 X SSC + 40 ml H2O, this solution can be used several times; the quality of the formamide is important, i. e., formamide from CMS is of constant good
tag on either the sense or antisense strand (City of Hope Protein and synthesis core facility) and various iterations were generated by first heating the RNAs (sense or antisense +/- biotin) to 70°C for 5 minutes and then mixed together and room
CGH of Biotin Labeled DNA vs Digoxigenin Labeled DNA
volume of 3M Na Acetate, mixing with pipet. Add 2.5 X (original) vol 100% EtOH to ppt DNA, vortex gently. Spin 30 mins at 14K rpm, 4 C. Decant supernatant; blot dry, being careful to avoid DNA pellet. Add 10 ul of MM1/H20 mix (70% MM1/30
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