Avidin PMMA Particles/PMMA微球,

Micro/Nanopatterning of Proteins Using a Nanoimprint-Based Contact Printing Technique

is achieved because of the homogeneous pressure applied via the nanoimprint lithography instrument, and it has allowed us to print lines of protein 150 nm wide, at a 400 nm period. This technique, therefore, provides an excellent method for the direct printing

RNA-biotin based pulldown assays for the detection of siRNA targeted genomic regions and siRNA directed histone modifications (PROT32)

nM 5'biotin end-labeled siRNA under gentle agitation, i.e. a rocking plate (Figure 1a). We utilized the EF1 alpha specific siRNA, EF52, shown previously to direct TGS in human cells (Morris et al. 2004). The EF52 siRNA was constructed with a 5' biotin

微流控芯片紫外检测仪的研制及性能考察

acid）为试样，对仪器的性能进行了考察。取浓度为1、5、10、50ppm的对氨基苯甲酸标准溶液进行芯片电泳检测，在浓度1到50ppm范围内有良好的线性关系，线性相关系数r=0.9984。此外，在对该仪器进行灵敏度考察时，其灵敏度达到0.1mg/L，与常规毛细管电泳仪的检测限接近。图2是浓度为1ppm的对氨基苯甲酸样品平行进行5次进样后得到的结果。检测波长选用270nm，缓冲液为30mM硼砂，进样电压为400V，进样时间为30秒，分离电压为600V，分离距离为47mm。 图