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- 文献和实验
- 技术资料
- 英文名:
Avidin PMMA Particles
- 库存:
100
- 供应商:
百欧泰(biotyscience)
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文献和实验nM 5'biotin end-labeled siRNA under gentle agitation, i.e. a rocking plate (Figure 1a). We utilized the EF1 alpha specific siRNA, EF52, shown previously to direct TGS in human cells (Morris et al. 2004). The EF52 siRNA was constructed with a 5' biotin
DNA (50ug/ml stock) + 2µl loading dye + 10.5µl sterile distilled water c. 50 ng/µl 1.0µl " + " + 10.0µl " c. 100 ng/µl 2.0µl " + " + 9.0µl " c. 200 ng/µl 4.0µl " + " + 7.0µl " 5) These tubes were given a short spin. 6) The samples were loaded
. ABTS Substrate Solution: 150 mg 2,2’-Azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) (Sigma, Cat. No. A-1888) Add to 500 ml of 0.1M citric acid in ddH2 O Adjust pH to 4.35 with NaOH Aliquot 11 ml per vial and store at -200
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