- 询价
- 百欧泰(biotyscience)
- 北京
- ABT-31-70000-Protein A
- 2026年04月12日
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- 详细信息
- 文献和实验
- 技术资料
- 英文名：
Protein A PMMA Particles
- 库存：
100
- 供应商：
百欧泰(biotyscience)
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文献和实验1:1 with Binding buffer.Filter through 0.45 um filters (use prefilters).(2) For Purification of Abs by Protein A columnPrepare Protein A column.Run binding buffer (pH9.0) ~ 200 ml.Filter protein mixtureAdd protein mixture or culture supernatant containing Ab
at the bottom of a vacuum tube under a stream of nitrogen. Suspend lipid film in 0.2 ml aqueous solution of purified porin or porin containing cell envelopes. It is best to use a concentrated protein solution ( E. coli porins: use 5-20 µg/200 µl P. aeruginosa porins: use
Falcon tubes to determine volume. Dilute the mixture 1:1 with Binding buffer . Filter through 0.45 um filters (use prefilters). For Purification of Abs by Protein A column: Prepare Protein A column. Run binding
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