- ￥2500
- 百欧泰(biotyscience)
- 北京
- ABT-31-0015-Protein A
- 2026年04月11日
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- 详细信息
- 文献和实验
- 技术资料
- 英文名：
Protein A PMMA Particles
- 库存：
100
- 供应商：
百欧泰(biotyscience)
- 规格：
10 mg/ml10ml
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文献和实验chloride,citrate,cysteine,diethanolamine,dithiothreitol,EDTA,EGTA,HEPES,mercaptoethanol,Nonidet P-40,phenol,polyvinyl pyrrolidone,sodium deoxycholate,sodium salicylate,thimerosol,Tricine,TRIS and Triton X-100.There is also much protein-to-protein variation
triton X (1% final concentration). Place tubes in rotary shaker at 4°C for 15 minutes. Spin cells for 15 minutes at 7000 RPM, collect supernatant into Beckman centrifuge tube. Spin again for 30 minutes at 45 K. Separate supernatant (our protein is soluble
Protein Syntheses in Cell Free Systems
spectrophotometer to measure the A .The extinction coefficient for ribosomes is 12 A units per mg per ml at 260 nm.14.The ribosomes may be frozen and stored in liquid nitrogen,or used for in vitro protein synthesis.If frozen,they should be thawed
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