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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
2-10℃
- 保质期：
以标签或说明书为准
- 英文名：
Sample Buffer Solution with 3-Mercapto-1,2-propanediol (×2)
- 库存：
9999
- 供应商：
广州威佳科技有限公司
- 规格：
25mL
6CbP1d
名称：Sample Buffer Solution with 3-Mercapto-1,2-propanediol (×2)（25mL）
E0C1N2p
规格：25mL
4J6O4a
109Y1Y9R9zI7Q7V2mP5U7W6z
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文献和实验might come out of solution, be aware of potential protease activity though.Hot SDS can be used to increase initial protein solubilisation. However, before electrophoresis it must be removed by competitive displacement in SD buffer. Post solubilisation TCA
results and will complicate analysis of the completed gel. Sample Solubilisation. The preparation of sample for 2D electrophoresis generally requires the use of Solubilisation/Denaturation (SD) buffer. This is used not only to separate the proteins
probe and 0.5 μg of poly(dI/dC) in 20 μl of 1X Binding buffer. Incubate at RT for 20 min. 4. Add 2 μl of loading dye, load total amount of the sample on 4% 0.25X TBE gel which has been pre-run for at least 20 min in 0.25X TBE buffer.
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