- 询价
- Biogradetech
- 美国
- BGT-ANT-37078
- 2025年07月11日
- ELISA,FCM,IHC
- Mouse
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
免疫原详情见datasheet，该抗体亚型为：IgG2a, kappa
- 保存条件：
-2°C-8°C
- 保质期：
1年
- 库存：
100
- 供应商：
艾美捷科技
- 宿主：
Mouse
- 应用范围：
ELISA,FCM,IHC
- 抗体英文名：
TF epitope/T-Ag Antibody
- 抗体名：
TF epitope/T-Ag Antibody
TF epitope/T-Ag Antibody，TF epitope/T-Ag Antibody，TF epitope/T-Ag Antibody,TF epitope/T-Ag Antibody
产品名称：TF epitope/T-Ag Antibody-TF epitope/T-Ag Antibody
产品货号：BGT-ANT-37078
产品规格：50ug#100ug#1mg
应用类型：ELISA,FCM,IHC
蛋白别名：T-Ag, TF-Ag, T antigen, TF epitope, Thomsen-Friedenreich epitope, β-D-Galp-(1→3)-D-GalpNAc, Galβ1-3GalNAc, (Gal)1 (GalNAc)1
TF epitope/T-Ag Antibody-TF epitope/T-Ag Antibody详情见产品datasheet
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文献和实验Monoclonal Antibody Production Protocol
1 - Substrate = ABTS - 100X (from Zymed 00-2001)1 - HRP buffer: 100mM Na Citrate pH 4.2( 490 mg citric acid + 720 mg Na citrate dehydrate + 50 ml H2O, pH 4.2 ). - Hydrogen peroxide 30% ( 1000X ) Protocol: ABSORBTION OF ANTIGEN Dilute Ag to 10 ug/ml in PBS
Notes on Making Rat x Y3 Monoclonal Antibody Producing Hybridomas
Although Y3/Ag1.2.3 has its own myeloma light chain, their are numerous characteristics that make this a better parent line for making hybridomas than the 'Y0' chain loss variants or using NS0 to make rat x mouse hybrids. These are: a) Hybridomas
cells at 3000 X g. Resuspend in 200 mls Solution A. Repeat wash. Pellet cells as above and resuspend in 4 mls Solution A. Reserve 2 mls of the cell suspension. Spheroplast the remaining 2 ml of the cell suspension by addition of beta
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