EZElisa™小鼠核纤层蛋白A/C（LMNA）ELISA试

（共享）ELISA原理与实验方法

, incubate for 30 min at 37°C. ( 5g powdered milk in 95 ml PBS)5 Rinse with PBS 3x.6 Add 50 µl of antibody, incubate 30 min at 37°C.7 Rinse with PBS 3x.8 Add 50 µl of goat anti-mouse IgG conjugate with alkaline phosphatase.(1 µl of conjugate in 4 ml of PBS

RNAse A Treatment of Mouse Cells

screwed up as the staining of several nuclear components (nuclear membrane protein lamin B; splicing factors Sc-35; centromeric proteins CENP-A, B, and C) is preserved. Back to top Materials & Reagents CSK buffer

ELISA with platelets

, dry 5. block half of the plate (with platelets) by PBS-BSA 1% and coat another half by glycine (x1) - BSA for 1 hour at 37ѓC 6. wash 3 times in PBS-tween 20, dry 7. incubated at 37ѓc for 1 hour with a 1:1000 dilution of rat IgG2a anti-mouse kappa