EZElisa ™ Allopregnanolone（AP）

在无血清细胞培养条件下将人 iPS 细胞体外分化为结肠类器官

类器官分化流程。人结肠类器官可以通过定形内胚层、后肠内胚层和结肠类器官扩增阶段，使用三步分化方案从人 iPS 细胞生成。 SCM302：定形内胚层诱导培养基，SCM303：后肠诱导培养基，SCM304：3dGRO™ 人结肠类器官扩增培养基 类器官培养方案 步骤1：人 iPS 细胞分化为定形内胚层（第 0-4 天） 注意：起始材料为高品质未分化的人 ES/iPS 细胞(SCC271)（细胞融合度约为 70-80%，且含有 准备单细胞传代培养基。将 ROCK 抑制剂 (ROCKi) Y

sothing about Genome walking

and get the bands that i want, do i clone them and sequence them using the same primers that i designed for the initial PCR reaction? Which vector should i use? Do you have any to recommend?Also, have you heard of Seegene's DNA Walking SpeedUp™ Premix Kit? I saw

Western Blotting Protocols

for primary antibody), labeled with alkaline phosphatase or horseradish peroxidase. Substrate appropriate to the enzyme conjugate (HRP or AP). Appropriate wash buffer: Phosphate-buffered saline (PBST): 10 mM sodium phosphate, pH 7.2, 0.9% (w/v